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RNAi: A Guide to Gene Silencing

Hardcover

ISBN-10:0-87969-641-9
ISBN-13:978-0-87969-641-2

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Description

General descriptionThe use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.

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publication infoG. Hannon, ed., Cold Spring Harbor Laboratory Press, 2003, 436 pp., hard cover

Table Of Contents

Table of ContentsChapter 1. Sense Cosuppression in Plants: Past, Present, and Future
Chapter 2. Transgene Cosuppression in Animals
Chapter 3. Regulation of the Genome by Double-stranded RNA
Chapter 4. RNAi in Caenorhabditis elegans
Chapter 5. Biochemistry of RNAi in Drosophila
Chapter 6. Stable Suppression of RNAi in Mammals
Chapter 7. RNAi Proteins and Functions in Plants: Genetics of RNAi in Plants
Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing
Chapter 9. RNA-dependent RNA Polymerase in Gene Silencing
Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization
Chapter 11. Total Interference: Genome-wide RNAi Screens in C. elegans
Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants
Chapter 13. Mammalian RNA Interference
Chapter 14. RNAi in Avian Embryos
Chapter 15. Guide to RNAi in Mouse Oocytes and Preimplantation Embryos
Chapter 16. RNAi Technologies in Drosophila Cell Culture
Chapter 17. RNAi Applications in Drosophila melanogaster
Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms